How should I prepare my libraries for Cloudbreak™ UltraQ™ Sequencing?

While developing UltraQ kits, Element BiosciencesTM researchers determined that the leading contributors to error in next generation sequencing occur during the library preparation process ( Q50+: Setting a New Standard for Sequencing Accuracy with Cloudbreak UltraQ ). The two main sources of library-induced error are deamination damage from steps requiring heat, and mismatched base incorporation during the end repair process. UltraQ sequencing kits address these issues by removing damaged fragments prior to circularization and omitting end-repair errors by dark cycling through the regions mos