Improving haloalkane dehalogenase thermostability by +20 °C while enhancing activity and expression

Scientists at Cradle optimized a haloalkane dehalogenase for thermostability and expression while preserving activity across two rounds. The best candidate achieved a melting temperature increase of +20.0 °C to 65.1 °C, 2.06× improved expression, and 1.29× enhanced activity.