Engineering polyspecific VHHs with picomolar binding to SARS-CoV-2 wild type and Omicron

Scientists at Cradle optimized a VHH for simultaneous binding to SARS-CoV-2 wild type and Omicron, thermostability, and expression across three rounds. The best candidate achieved a K_D of 186 pM against wild type, 11.4 nM against Omicron, a melting temperature of 70.9 °C, and 1.88× improved expression.