Engineering polyspecific anti-venom VHHs with sub-nanomolar binding and high thermostability

Scientists using Cradle optimized VHHs for polyspecific binding to three distinct snake venom neurotoxins, thermostability, and expression across two rounds. The best candidate achieved binding below 100 pM, below 1 nM, and below 2 nM to the three toxins respectively, with a melting temperature of 76.7 °C and 1.59× improved expression.